Job Description
The incumbent will perform protein purification and characterization from various expression systems.
Key Responsibilities:
Independently perform protein purification from various expression systems (Mammalian, E. coli & Insect cell) and QC.
Prepare detailed reports for sharing with clients and attend telecons.
Improve lab efficiency and streamlines the operational processes to increase productivity.
Analyze operational problems, identify solutions, and implement corrective actions.
Guide and train research scientists in the team at various levels.
Follow environment, health, and safety (EHS) requirements at all times in the workplace ensuring individual and lab/plant safety
Attend training on environment, health, and safety (EHS) measures imparted company
Educational Qualification:
M.Sc/ M.Tech/MS (Biotechnology, Biochemistry or a related discipline)
Technical and Functional skills:
Expertise in routine protein purification with affinity, size exclusion, ion exchange chromatography using AKTA FPLC systems (Unicorn software).
Expertise in protein purification of antibodies, affinity-tagged, secreted proteins as well as "problematic" proteins (low expression, poor solubility, intra-cellular expression, prone to aggregation).
Proficiency in protein extraction and solubilization methods, protein refolding and purification via affinity, hydrophobic interaction (HIC), ion exchange (IEX), size exclusion (SEC) and mixed-mode chromatography.
Expertise in routine characterization of proteins including SDS-PAGE, SE-HPLC, and biophysical techniques.
Familiarity with mammalian and microbial protein expression is a plus
Experience:
5-7 years of relevant experience in Biotech/Pharma/R&D
Behavioral Skills:
Be polite, assertive and collaborate well with colleagues
Take accountability and ownership for respective job function
Good verbal and written communication skills